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General info and notes before starting

There are 13 water bath tubes set up to examine 13 DSPR founder lines of drosophila melanogaster:

Lines	Vial Code/Stock Number	Stock Name	# of Vials received 2018-06-12
AB8	Sam	n/a	3
A5	3875	VAG1	2
A7	T.7/14021-0231.7	n/a	2
A2	3841	BOG1	2
B6	T.1/14021-0231.1	n/a	2
B7	T.4/14021-0231.4	n/a	2
A4	3852	KSA2	2
A6	3886	wild5B	2
A3	3844	BS1	2
B2	3846	CA1	2
B3	3864	QI2	2
A1	1	Canton-S	2
B4	3870	RVC3	2

Machines, Apparatus, & Instruments

Water Bath: LAUDA ECO Silver Immersion thermostat, Model ECO RE 1050 S

Thermocoupler: Omega HH506RA Multilogger Thermometer

Glass Test tubes: Pyrex No. 9826

Test tube rack: Full-View Test Tube Rack, 13-16mm tubes, 50 Place

Tube Set Up: Cut half of a styrofoam falcon tube rack and hot clue to plastic test tube rack

Aspirator Set up

* Using the WinTherm-Thermal Analytics Software, set parameters as below

Temp (ºC)	Time (min)	Pump
25.1	5	2
25.2	1	2
25.3	1	2
25.4	1	2
25.5	1	2
25.6	1	2
25.7	1	2
25.8	1	2
25.9	1	2
26.0	1	2
26.1	1	2
26.2	1	2
26.3	1	2
26.4	1	2
26.5	1	2
26.6	1	2
26.7	1	2
26.8	1	2
26.9	1	2
27.0	1	2
27.1	1	2
27.2	1	2
27.3	1	2
27.4	1	2
27.5	1	2
27.6	1	2
27.7	1	2
27.8	1	2
27.9	1	2
28.0	1	2
28.1	1	2
28.2	1	2
28.3	1	2
28.4	1	2
28.5	1	2
28.6	1	2
28.7	1	2
28.8	1	2
28.9	1	2
29.0	1	2
29.1	1	2
29.2	1	2
29.3	1	2
29.4	1	2
29.5	1	2
29.6	1	2
29.7	1	2
29.8	1	2
29.9	1	2
30.0	1	2
30.1	1	2
30.2	1	2
30.3	1	2
30.4	1	2
30.5	1	2
30.6	1	2
30.7	1	2
30.8	1	2
30.9	1	2
31.0	1	2
31.1	1	2
31.2	1	2
31.3	1	2
31.4	1	2
31.5	1	2
31.6	1	2
31.7	1	2
31.8	1	2
31.9	1	2
32.0	1	2
32.1	1	2
32.2	1	2
32.3	1	2
32.4	1	2
32.5	1	2
32.6	1	2
32.7	1	2
32.8	1	2
32.9	1	2
33.0	1	2
33.1	1	2
33.2	1	2
33.3	1	2
33.4	1	2
33.5	1	2
33.6	1	2
33.7	1	2
33.8	1	2
33.9	1	2
34.0	1	2
34.1	1	2
34.2	1	2
34.3	1	2
34.4	1	2
34.5	1	2
34.6	1	2
34.7	1	2
34.8	1	2
34.9	1	2
35.0	1	2
35.1	1	2
35.2	1	2
35.3	1	2
35.4	1	2
35.5	1	2
35.6	1	2
35.7	1	2
35.8	1	2
35.9	1	2
36.0	1	2
36.1	1	2
36.2	1	2
36.3	1	2
36.4	1	2
36.5	1	2
36.6	1	2
36.7	1	2
36.8	1	2
36.9	1	2
37.0	1	2
37.1	1	2
37.2	1	2
37.3	1	2
37.4	1	2
37.5	1	2
37.6	1	2
37.7	1	2
37.8	1	2
37.9	1	2
38.0	1	2
38.1	1	2
38.2	1	2
38.3	1	2
38.4	1	2
38.5	1	2
38.6	1	2
38.7	1	2
38.8	1	2
38.9	1	2
39.0	1	2
39.1	1	2
39.2	1	2
39.3	1	2
39.4	1	2
39.5	1	2
39.6	1	2
39.7	1	2
39.8	1	2
39.9	1	2
40.0	200	2

Submerge apparatus into water bath.
Press start on the WinTherm-Thermal Analytics Software and click OK to confirm.
Check the flies once an hour for 2 hours. Once the temperature reaches 38.5ºC, observe fly knockdown.

* Using the WinTherm-Thermal Analytics Software, set parameters as below

Temp (ºC)	Time (min)	Pump
25	5	2
26	1	2
27	1	2
28	1	2
29	1	2
30	1	2
31	1	2
32	1	2
33	1	2
34	1	2
35	1	2
36.5	1	2
37.5	1	2
38.5	1	2
40	200	2

Using the WinTherm-Thermal Analytics Software, imput desired temperature into “set temp” box and press enter.
Submerge apparatus into water bath for 200 minutes.

Using the WinTherm-Thermal Analytics Software, input first desired temperature into “set temp” box and press enter.
Submerge apparatus into water bath for 60 minutes.
Remove the apparatus for 5 minutes
Input second desired temperature into “set temp” box and press enter.
Re-insert the apparatus into the water bath for 200min.